ABSTRACT
@#To explore the effects of extract of selenium-enriched Astragalus membranaceus(ESAM)on insulin resistance(IR)in streptozotocin(STZ)diabetic rats. In this study, type 2 diabetes mellitus(T2DM)was established; ESAM and pioglitazone were used to intervene T2DM model rats; the general condition of rats in each group was observed, and body weight and fasting blood glucose(FBG)were recorded before modeling and after modeling. At the end of the fourth week, the blood and liver tissues of each group were collected. The total cholesterol(CHOL), triglyceride(TG), high-density lipoprotein(HDL), low-density lipoprotein(LDL)in blood were detected by biochemical detector; ELISA was used to detected content changes of blood fasting insulin(FINS), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), C-reactive protein(CRP), interleukin-1β(IL-1β); Western blotting was used for the detection of insulin receptor substrate protein 1(IRS1), phosphorylated IRS1(p-IRS1), nuclear factor κB inhibitor kinase β(IKKβ), phosphorylated IKKβ(p-IKKβ), c -Jun N-terminal kinase(JNK), phosphorylated JNK(p-JNK)protein changes. Results showed that compared with the blank group, the body weight of the model group was significantly decreased. FBG, CHOL, TG, LDL, TNF-α, IL-6, CRP, IL-1β in the blood and p-IRS1, p-IKKβ, p-JNK in the liver was increased significantly; after intervention, pioglitazone, ESAM can reverse the body weight of the model rats and the changes of the above cytokines and proteins. In conclusion, ESAM can reduce the expression of inflammatory cytokines in T2DM rats, inhibit the increase of free fatty acids(FFA), decrease the activation of IKKβ and JNK phosphorylation in surrounding tissues, activate insulin pathway and improve the IR effect of T2DM.
ABSTRACT
To investigate the effects of lidocaine on phospholipase A_2(PLA_2) and fluidity of lung cell membrane during rabbits endotoxemia. Method: Twenty-four white rabbits were randomly assigned to receiving one of three treatmemnts (n=8 for each group): infusion of saline(control group), infusion of Escbericbia coli endotoxin(endotoxin group), infusion of endotoxin with lidocaine pretreatment (lidocaine group). The PLA_2 activity, PS, PE and PC contents of lung cell membrane fluidity were analysed with high performance liquid chromatography and DPH method respectively. Result: PLA_2 activities in the plasma and cell membrane were reduced significantly, and the PS, PE and PC contents in the cell membrane increased significantly in lidocaine group compared with those in endotoxin group. The membrane fluidities of RBC and lung cells of rabbit with lidocaine pretreatment also incresed significantly in comparison with those in endotoxin group. Conclusion:The pretreatment with lidocaine inhibits PLA_2 activity in plasma and cell membrane of rabbit lung, reduces the contents of phospholipids in cell membrane of rabbit lung, increases membrane fluidity of RBC and lung ceils of rabbit and stabilizes cell membranes function.